This article is from Scientific Reports, volume 3.AbstractThe integral membrane-bound Nrf1 transcription factor fulfils important functions in maintaining cellular homeostasis and organ integrity, but how it is controlled vectorially is unknown. Herein, creative use of Gal4-based reporter assays with protease protection assays (GRAPPA), and double fluorescence protease protection (dFPP), reveals that the membrane-topogenic vectorial behaviour of Nrf1 dictates its post-translational modification... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684815
byZhang, Yiguo; Ren, Yonggang; Li, Shaojun; Hayes, John D.
This article is from PLoS ONE, volume 9.AbstractThe membrane-bound Nrf1 transcription factor regulates critical homeostatic and developmental genes. The conserved N-terminal homology box 1 (NHB1) sequence in Nrf1 targets the cap‘n’collar (CNC) basic basic-region leucine zipper (bZIP) factor to the endoplasmic reticulum (ER), but it is unknown how its activity is controlled topologically within membranes. Herein, we report a hitherto unknown mechanism by which the transactivation activity of... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973704
This article is from PLoS ONE, volume 9.AbstractDespite androgen deprivation therapy (ADT), persistent androgen receptor (AR) signaling enables outgrowth of castration resistant prostate cancer (CRPC). In prostate cancer (PCa) cells, ADT may enhance AR activity through induction of oxidative stress. Herein, we investigated the roles of Nrf1 and Nrf2, transcription factors that regulate antioxidant gene expression, on hormone-mediated AR transactivation using a syngeneic in vitro model of... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899380